DEPD5 Antibody

• Catalog Number: orb1271048
• Category: Antibodies
• Description: DEPD5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids from the C-terminal region of human DEPD5.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 181 kDa
• Target: DEPDC5
• UniProt ID: O75140
• NCBI: O75140
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: DEP domain-containing protein 5, DEPDC5, KIAA0645
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of DEPD5 Antibody Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in K562 cell line lysate.