Deoxypodophyllotoxin

SKU: orb1220013

Description

Deoxypodophyllotoxin shows cytotoxic , antineoplastic, antitumor, insecticidal, anti-angiogenic, vascular disrupting, insecticidal, antiviral, and anti-inflammatory activities.Deoxypodophyllotoxin (DPT) induced both apoptosis and autophagy via production of mitochondrial reactive oxygen species (ROS). DPT suppressed the PI3K/AKT/mTOR signaling cascades to lead autophagy process, resulting from conversion of light chain 3-I (LC3-I) into LC3-II and acidic vesicular organelles (AVOs) formation. Even if DPT-induced ROS were occurred in both apoptosis and autophagy, inhibition of ROS generation enhanced cell viability. Otherwise, 3-methyladeine (3-MA) impeding on autophagy accelerated an apoptotic response caused by DPT. Therefore, these findings suggest that DPT triggers cytoprotective autophagy against cytotoxic apoptosis.

Key Properties

• CAS Number: 19186-35-7
• MW: 398.41
• Purity: >98% (HPLC)
• Formula: C₂₂H₂₂O₇
• SMILES: [H][C@]12COC(=O)[C@]1([H])[C@H](c1cc(OC)c(OC)c(OC)c1)c1cc3OCOc3cc1C2
• Target: Microtubule/Tubulin
• Solubility: In Vitro: DMSO : 100 mg/mL (251.00 mM)

Bioactivity

• In Vivo:
  Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively. Animal model: Xenograft model of gastric cancer in nude mice with SGC-7901 cells. Dosage: 5, 10, and 20 mg/kg. Administration: Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days. Result: Inhibited the growth of gastric cancer tumors.
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• In Vitro:
  Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell populationfrom 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively. Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners. Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity. Apoptosis Analysis Cell line: SGC-7901 cells. Concentration: 25, 50, 75 nM. Incubation time: 6, 12, 24, 48 hours. Result: Induced apoptosis in SGC-7901 Cells. Cell Cycle Analysis Cell line: SGC-7901 cells. Concentration: 25, 50, 75 nM. Incubation time: 6, 12, 24, 48 hours. Result: Induced G2/M cell cycle arrest in SGC-7901 Cells Western blot analysis. Cell line: SGC-7901 cells. Concentration: 25, 50, 75 nM. Incubation time: 6, 12, 24, 48 hours. Result: Altered the expression of cyclin B1, Cdc2, Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Microtubule/Tubulin