DENND4C Antibody (HRP)

SKU: orb699430

Description

Mouse monoclonal antibody against DENND4C conjugated to HRP

Research Area

Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IP, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SMC-610 was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 300000000
• Immunogen: Full Length Human Recombinant Dennd4c protein
• Target: DENND4C
• Molecular Weight: 213 kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DENN domain-containing protein 4C

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI.

Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody. Three amounts of (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C.

Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

DENND4C

UniProt

Q5VZ89

NCBI Gene ID

55667

RefSeq (mRNA):NM_017925