DENND4C Antibody: Biotin

• Catalog Number: orb699428
• Category: Antibodies
• Description: Mouse monoclonal antibody against DENND4C conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 300000000
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Full Length Human Recombinant Dennd4c protein
• Concentration: 1mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 213 kDa
• Target: DENND4C
• Entrez: 55667
• UniProt ID: Q5VZ89
• NCBI: NM_017925
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: DENN domain-containing protein 4C antibody
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-610 was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI.

Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody. Three amounts of (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C.

Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8. Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:1000.