DELTA-4 Antibody

SKU: orb345481

Description

DELTA-4 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, IHC: 10 µg/mL to 20 µg/mL, WB: 1:500 - 1:2,500
• Reactivity: Human, Mouse
• Application Notes:
  Anti-DELTA-4 has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 74 kDa in size corresponding to DELTA-4 by western blotting in the appropriate cell lysate or extract. For best results use Goat Serum as blocking agent in western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
• Purity: Anti-DELTA-4 affinity purified antibody is directed against human DELTA-4. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse, rat and chimpanzee DELTA-4 based on a 91% homology to the core 12 amino acid sequence within the 14 amino acid immunogen sequence. Cross reactivity with DELTA-4 homologues from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-DELTA4 antibody, DELTA-4, delta 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor, DELTA 4

ELISA results of purified Rabbit anti-DELTA-4 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Biorbyt's Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.

Western Blot of Rabbit anti-DELTA-4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate (p/n orb348719). Lane 2: HUVEK Whole Cell Lysate. Lane 3: Human rDLL4. Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate (p/n orb348669). Load: 10 µg per lane for Whole Cell Lysates or 50 ng for recombinant protein. Primary antibody: DELTA-4 antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 30 min at RT. Predicted/Observed size: 74 kDa, 74 kDa for DELTA-4. Other band(s): Not Identified.

Western Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue (p/n orb348719). Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody (p/n orb347654) at 1:5000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.

Quick Database Links

UniProt

Q9NR61

RefSeq:9506545