DEFB121 Polyclonal Antibody

• Catalog Number: orb1420149
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DEFB121.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• UniProt ID: Q5J5C9
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Beta-defensin 121, Beta-defensin 21, DEFB-21, Defe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DEFB121) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.