DEDAF Antibody

• Catalog Number: orb1239322
• Category: Antibodies
• Description: DEDAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF.The immunogen is located within the last 50 amino acids of DEDAF.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: RYBP
• UniProt ID: Q8N488
• NCBI: AF179286
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-
• Note: For research use only
• Application notes: DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of DEDAF in mouse liver tissue with DEDAF antibody at 20 µg/ml.

Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 µg/mL.

Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 µg/mL.

Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/mL.

Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 µg/ml.