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Catalog Number | orb1255736 |
---|---|
Category | Antibodies |
Description | DDX50 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1). |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 105kDa |
Target | DDX50 |
UniProt ID | Q9BQ39 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | DDX50, GU2, GUB, mcdrh, RH-II/GuB Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DDX50 antibody (orb1255736) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (orb1255736) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (orb1255736) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.
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