DDX41 Antibody

SKU: orb1256921

Description

DDX41 Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
• Target: DDX41
• Molecular Weight: Observed: 75kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

DDX41 Antibody: ABS, ABS, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41

Western blot analysis of extracts of various cell lines, using DDX41 antibody (orb1256921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using DDX41 antibody (orb1256921). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug DDX41 antibody (orb1256921). Western blot was performed from the immunoprecipitate using DDX41 antibody (orb1256921) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

DDX41

UniProt

Q9UJV9