DDX39B Antibody

SKU: orb1255890

Description

DDX39B Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 319-428 of human DDX39B (NP_004631.1).
• Target: DDX39B
• Molecular Weight: Observed: 49kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

BAT1, BAT1, UAP56, D6S81E

Western blot analysis of extracts of various cell lines, using DDX39B antibody (orb1255890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat liver using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using DDX39B antibody (orb1255890) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using DDX39B Polyclonal Antibody (orb1255890) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using DDX39B Polyclonal Antibody (orb1255890) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using DDX39B Polyclonal Antibody (orb1255890) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DDX39B

UniProt

Q13838