DDX27 Antibody

• Catalog Number: orb1270377
• Category: Antibodies
• Description: DDX27 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-796 amino acids from the C-terminal region of human DDX27.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90 kDa
• Target: DDX27
• UniProt ID: Q96GQ7
• NCBI: Q96GQ7
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Probable ATP-dependent RNA helicase DDX27, DEAD bo
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in K562 cell line lysates (35 ug/lane).

DDX27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.