Rabbit DDX27 Antibody

SKU: orb1527012

Description

DDX27 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended.; IP - 5 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 700 and 750
• Target: DDX27
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DEAD (Asp-Glu-Ala-Asp) box polypeptide 27; DEAD box protein 27; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27; Deficiency of ribosomal subunits protein 1 homolog; dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241; probable ATP-dependent RNA helicase DDX27; RHLP; RNA helicase-like protein

Detection of human DDX27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX27 antibody orb1527012 used for IP at 10 µg/mg lysate.

Detection of human and mouse DDX27 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DDX27 (Cat. No. orb1527012) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

DDX27

UniProt

Q96GQ7

RefSeq (Protein):NP_060365.6