DDX27 Rabbit Polyclonal Antibody

SKU: orb2563524

Description

DDX27 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DDX27 (61-160/796aa)
• Target: DDX27
• Molecular Weight: 90 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C86129; cb843; DDX27; DDX27_HUMAN; DEAD(Asp-Glu-Ala-Asp) box polypeptide 27; DEAD box protein 27; Deficiency of ribosomal subunits protein 1 homolog; dJ686N3.1; DKFZp667N057; DRS1; FLJ12917; FLJ20596; FLJ22238; HSPC259; MGC1018; MGC163147; PP3241; Probable ATP-dependent RNA helicase DDX27; RHLP; RNA helicase like protein; Rrp3p.

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-DDX27 antibody (orb2563524), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (DDX27) polyclonal Antibody, Unconjugated (orb2563524) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

DDX27

UniProt

Q96GQ7