DDX17 (vPairTM) Antibodies

SKU: orb131829

Description

Rabbit polyclonal to DDX17. DDX17 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Probable ATP-dependent RNA helicase DDX17 belongs to the DEAD box helicase family and the DDX5/DBP2 subfamily. DDX17 is an RNA-dependent ATPase activity. DDX17 is involved in transcriptional regulation and is a transcriptional coactivator for estrogen receptor ESR1. DDX17 increases ESR1 AF-1 domain-mediated transactivation. Additionally, DDX17 synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and is probably involved in skeletal muscle differentiation. Furthermore, DDX17 is required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation.

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human, Mouse
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [DDX17 (N) (R1762-1)] or the C-terminal [DDX17 (C) (R1762-2)] region of human DDX17.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or the C-terminal region of human DDX17
• Molecular Weight: 80
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Probable ATP-dependent RNA helicase DDX17 antibody pairs, anti DEAD box protein 17 antibody pairs, anti DEAD box protein p72 antibody pairs, anti RNAdependent helicase p72 antibody pairs

Quick Database Links

UniProt

Q92841