DDH/AKR1C1 Antibody

• Catalog Number: orb1535218
• Category: Antibodies
• Description: DDH/AKR1C1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IHC-P, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
• Concentration: 0.6 mg/ml
• Dilution range: ICC (1:100 - 1:1000), IHC, IHC-P (7.5 µg/ml), WB (1:500 - 1:3000)
• Conjugation: Unconjugated
• Target: DDH / AKR1C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7, 0.025% ProClin 300, 20% Glycerol
• Alternative names: AKR1C1, 2-ALPHA-HSD, 20-alpha-HSD, Aldo-keto reduc
• Note: For research use only
• Application notes: Further information: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0).
• Expiration Date: 12 months from date of receipt.

Anti-DDH / AKR1C1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunofluorescence of methanol-fixed A431 using AKR1C1 antibody at 1:200 dilution.

Western blot analysis of Anti-AKR1C1 antibody (orb1535218, 1:1000 dilution; 50 µg of lysate/extract per lane). Lane 1: Mouse liver lysate/extract. Antibody produced band at ~37 kDa.