DDH/AKR1C1 Antibody

SKU: orb1535218

Description

DDH/AKR1C1 Antibody

Images & Validation

• Tested Applications: ICC, IHC, IHC-P, WB
• Dilution Range: ICC (1:100 - 1:1000), IHC, IHC-P (7.5 µg/ml), WB (1:500 - 1:3000)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0).

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
• Target: DDH / AKR1C1
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7, 0.025% ProClin 300, 20% Glycerol
• Concentration: 0.6 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKR1C1, 2-ALPHA-HSD, 20-alpha-HSD, Aldo-keto reductase C, Bile acid binding protein, Chlordecone reductase homolog, DDH1, Dihydrodiol dehydrogenase 2, DD1, DDH, Dihydrodiol dehydrogenase 1/2, DD1/DD2, Dihydrodiol dehydrogenase 1, H-37, HAKRC, HBAB, Indanol dehydrogenase, MBAB

Anti-DDH / AKR1C1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.

Immunofluorescence of methanol-fixed A431 using AKR1C1 antibody at 1:200 dilution.

Western blot analysis of Anti-AKR1C1 antibody (orb1535218, 1:1000 dilution; 50 µg of lysate/extract per lane). Lane 1: Mouse liver lysate/extract. Antibody produced band at ~37 kDa.

Quick Database Links

Gene Symbol

DDH / AKR1C1