DDB2 Antibody

• Catalog Number: orb1258426
• Category: Antibodies
• Description: DDB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 48kDa
• Target: DDB2
• UniProt ID: Q92466
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: DDBB, UV-DDB2, XPE, DNA damage-binding protein 2,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DDB2 antibody (orb1258426) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF-7 cells using DDB2 antibody (orb1258426). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using DDB2 antibody (orb1258426). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.