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DDAH2 Rabbit pAb Discontinued

SKU: orb247451

Description

Rabbit polyclonal antibody to DDAH2

Images & Validation

Tested ApplicationsELISA, IF, IHC-P, WB
Dilution RangeWB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:10-100
ReactivityHuman, Mouse, Rat

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH2 (NP_039268.1).
Molecular Weight30kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide,50% glycerol,pH7.3.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti G6a antibody, anti DDAH antibody, anti NG30 antibody, anti DDAHII antibody, anti HEL-S-277 antibody

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DDAH2 Rabbit pAb

Immunofluorescence analysis of U2OS cells using DDAH2 antibody (orb247451). Blue: DAPI for nuclear staining.

DDAH2 Rabbit pAb

Western blot analysis of extracts of various cell lines, using DDAH2 antibody (orb247451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

DDAH2 Rabbit pAb (orb247451)

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