DCX Antibody

SKU: orb1249258

Description

DCX Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03µg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KTSANMKAPQS
• Target: DCX
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin

orb1249258 (0.01 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249258 (2 ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1249258 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

orb1249258 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

Quick Database Links

Gene Symbol

DCX

UniProt

O43602

RefSeq (Protein):NP_000546.2, NP_835365.1, NP_001182482.1, NP_835364.1