DCUN1D1 Antibody

SKU: orb1249142

Description

DCUN1D1 Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in HepG2 lysates (calculated MW of 30.1kDa according to NP_065691.2). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:In paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Recommended concentration, 5-10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RPQIAGTKSTT
• Target: DCUN1D1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCUN1D1, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae), DCUN1L1, RP42, SCCRO, SCRO, Tes3, RP42 homolog, squamous cell carcinoma-related oncogene

orb1249142 (0.5 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249142 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

DCUN1D1

UniProt

Q96GG9

RefSeq (Protein):NP_065691.2