DCUN1D1 Antibody

• Catalog Number: orb1249142
• Category: Antibodies
• Description: DCUN1D1 Antibody
• Target: DCUN1D1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-RPQIAGTKSTT
• UniProt ID: Q96GG9
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in HepG2 lysates (calculated MW of 30.1kDa according to NP_065691.2). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DCUN1D1, DCN1, defective in cullin neddylation 1,
• Research Area: Cancer Biology, Neuroscience
• Note: For research use only
• NCBI: NP_065691.2

orb1249142 (0.5 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249142 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.