DCLK1 Antibody

SKU: orb1239319

Description

DCLK1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  DCLK1 antibody can be used for detection of DCLK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK1.The immunogen is located within the last 50 amino acids of DCLK1.
• Target: DCLK1
• Molecular Weight: Predicted: 90 kDa Observed: 85 kDa
• Purification: DCLK1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: DCLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCLK1 Antibody: CL1, DCLK, CLICK1, DCDC3A, DCAMKL1, KIAA0369, Doublecortin domain-containing protein 3A

Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 µg/mL.

Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 µg/mL.

Immunofluorescence of DCLK1 in mouse brain tissue with DCLK1 Antibody at 20 µg/mL. Green: DCLK1 Antibody (orb1239319) Blue: DAPI staining.

Immunohistochemistry of DCLK1 in mouse brain tissue with DCLK1 Antibody at 5 µg/mL.

Western blot analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 µg/ml.

Quick Database Links

Gene Symbol

DCLK1

UniProt

O15075

RefSeq (Protein):NP_004725