DCDC2 Antibody

• Catalog Number: orb1247619
• Category: Antibodies
• Description: DCDC2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-DGEKANKDAEQKED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52kDa band observed (calculated MW of 52.8kDa according to NP_057440.1). Data kindly provided by Wang and LoTurco, University of Connecticut, USA. Recommmended concentration 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Mouse brain tissue.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10ug/ml. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DCDC2
• UniProt ID: Q9UHG0
• NCBI: NP_057440.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DCDC2, doublecortin domain containing 2, HGNC:1814
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Full length recombinant human DCDC2 expressed in COS7 (Lane A), non-transfectant COS7 (lane B). Approx 52kDa band observed (calculated MW of 52.8kDa according to NP_057440.1). Data kindly provided by Wang and LoTurco, University of Connecticut, USA. Recommmended concentration 0.1-0.3ug/ml. An anonymous customer found positive results in WB on Mouse brain tissue.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in select neurons. Recommended concentration, 5-10ug/ml. An anonymous customer found positive results in IHC on Rat retinal tissue. An anonymous customer found positive results in IHC on Rat dorsal root ganglion.
• Expiration Date: 12 months from date of receipt.

orb1247619 (0.2 ug/ml) staining of COS7 cell lysate transfected with full length recombinant human DCDC2 (A) and untransfected control COS7 cells (B). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247619 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.