DCAF7 Antibody

SKU: orb1256773

Description

DCAF7 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:10 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
• Target: DCAF7
• Molecular Weight: Observed: 39kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DDB1- and CUL4-associated factor 7, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog, DCAF7, HAN11, WDR68

Western blot analysis of extracts of 293T cells, using DCAF7 antibody (orb1256773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF7 cells using DCAF7 antibody (orb1256773). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DCAF7

UniProt

P61962