DC-SIGN/CD209 Antibody

• Catalog Number: orb318734
• Category: Antibodies
• Description: Goat polyclonal antibody to CD209
• Target: DC-SIGN / CD209
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NRGEPNNVGEED
• MW: 45.8; 43.3; 45.0; 30.5; 41.0; 35.2; 27.4
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Application notes: WB: Approx 50kDa band observed in Human Bone Marrow lysates (calculated MW of 45.8kDa according to NP_066978.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD209 antibody, anti CD209 molecule antibody,
• Note: For research use only
• Entrez: 30835

1 µg/ml staining of K562 (A) and (2 ug/ml) THP-1 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

6 µg/ml staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

Flow cytometric analysis of paraformaldehyde fixed THP1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 immobilized on Shi-fix™ plus cover-slips. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.