DBCO-PEG4-FLAG

SKU: orb1734976

Description

DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 M) or labeling of Azide-functionalized micelles (amount: 100 nmol). These concentrations may serve as a starting point for individual assay set-up.

Images & Validation

• Application Notes:
  Spectroscopic Propertie: max 308 nm, 13.8.0 L mmol-1cm-1.

Key Properties

• MW: Theoretical MW: 1790.85 g/mol; Detected MW: 1789.70 g/mol
• Purity: 95% (HPLC)
• Formula: C₈₀H₁₀₇N₁₅O₃₀S
• Solubility: water, PBS (up to 30 mM tested)

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dibenzylcyclooctyne-PEG4-FLAGtag (DYKDDDDK)