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| Catalog Number | orb532287 |
|---|---|
| Category | Tools |
| Description | DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 µM) or labeling of Azide-functionalized micelles (amount: 100 nmol). These concentrations may serve as a starting point for individual assay set-up. |
| Form/Appearance | solid; Color: white to off-white |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 1790.85 g/mol; Detected MW: 1789.70 g/mol |
| Formula | C80H107N15O30S |
| Solubility (25°C) | water, PBS (up to 30 mM tested) |
| Storage | store at -20 °C |
| Note | For research use only |
| Application notes | < b>Spectroscopic Propertie: λmax 308 nm, ε 13.8.0 L mmol-1cm-1. |
| Expiration Date | 12 months from date of receipt. |
