Daunorubicin hydrochloride

SKU: orb1225564

Description

Daunorubicin is potent topoisomerase II (Topo II) inhibitor, interacts with DNA by intercalation and inhibition of macromolecular biosynthesis in cancer cells; Daunorubicin is a chemotherapy agent used to treat multiple cancer, secifically used for acute myeloid leukemia (AML), acute lymphocytic leukemia (ALL), chronic myelogenous leukemia (CML), and Kaposi's sarcoma; Daunorubicin and it's derivatives are widely used as payloads in antibody-drug conjugates (ADCs).Chemotherapeutic Agents Approved(In Vitro):Daunorubicin hydrochloride (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells.Daunorubicin hydrochloride (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells.Daunorubicin hydrochloride (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells.Daunorubicin hydrochloride (0.4 μM, 120 min) induces ROS generation in L3.6 cells.Daunorubicin hydrochloride (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line).(In Vivo):Daunorubicin hydrochloride (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats.Daunorubicin hydrochloride (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 23541-50-6
• MW: 563.9808
• Purity: >98% (HPLC)
• Formula: C₂₇H₃₀ClNO₁₀
• SMILES: C[C@H]1[C@H]([C@H](C[C@@H](O1)O[C@H]2C[C@@](CC3=C(C4=C(C(=C23)O)C(=O)C5=C(C4=O)C=CC=C5OC)O)(C(=O)C)O)N)O.Cl
• Target: ADC Cytotoxin
• Solubility: H2O: ≥ 34 mg/mL

Bioactivity

• In Vivo:
  Daunorubicin hydrochloride (intravenous injection, 3 mg/kg, three times at 48 h intervals.) produces cardiotoxicity and nephrotoxicity in rats. Daunorubicin hydrochloride (intraperitoneal injection, 10 mg/kg) induces sister chromatid exchanges in mice. Animal model: Male Sprague-Dawley rats. Dosage: 3 mg/kg. Administration: Intravenous injection, three times at 48 h intervals. Result: Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity. Increased urinary protein excretion, serum creatinine, and BUN level.
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• In Vitro:
  Daunorubicin hydrochloride (0-256 μg/mL, 30 min) inhibits DNA and RNA synthesis in sensitive and resistant Ehrlich ascites tumor cells. Daunorubicin hydrochloride (7 nM-1.9 μM, 72 h) shows chemosensitivity in Molt-4 cells and L3.6 cells. Daunorubicin hydrochloride (0.4 μM, 48 h) induces apoptotic and necrosis in L3.6 cells. Daunorubicin hydrochloride (0.4 μM, 120 min) induces ROS generation in L3.6 cells. Daunorubicin hydrochloride (2 μM, 24 h) induces autophagy in K562 cells (myeloid cell line). Cell Viability Assay Cell line: Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line) Concentration: 7 nM-1.9 μM Incubation time: 72 h. Result: Inhibited cell viability with IC50 values of 40 nM (Molt-4) and 400 nM (L3.6). Apoptosis Analysis Cell line: L3.6 cells Concentration: 0.4 μM Incubation time: 72 h. Result: Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h. Western blot analysis: Cell line: K562 cells. Concentration: 2 μM. Incubation time: 24 h. Result: Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RP 13057 Hydrochloride | Daunomycin | RP13057 Hydrochloride

Quick Database Links

Gene Symbol

ADC Cytotoxin