DARPP-32 (phospho Thr75) rabbit pAb Antibody

SKU: orb764172

Description

Rabbit polyclonal antibody to DARPP-32 (phospho-Thr75)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. AA range:41-90
• Molecular Weight: 32
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PPP1R1B antibody, anti-DARPP32 antibody, anti-Protein phosphatase 1 regulatory subunit 1B antibody, anti-DARPP-32 antibody, anti-Dopamine- and cAMP-regulated neuronal phosphoprotein antibody

Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.

Western Blot analysis of various cells using Phospho-DARPP-32 (T75) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.