DAPK3/ZIP Kinase Antibody

• Catalog Number: orb1538593
• Category: Antibodies
• Description: DAPK3/ZIP Kinase Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within aa 270-320 of ZIPK.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:1000)
• Conjugation: Unconjugated
• Target: DAPK3 / ZIP Kinase
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: DAPK3, DAP-like kinase, Dlk, MYPT1 kinase, ZIP kin
• Note: For research use only
• Application notes: Further information: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 ug/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 26 kDa Observed: 27 kDa
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 µg/mL.

Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 ug/ml.