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Catalog Number | orb1929471 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB3033 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 160046 Da |
Target | This DAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1360-1389 amino acids from the C-terminal region of human DAPK1. |
UniProt ID | P53355 |
NCBI | NP_004929.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Death-associated protein kinase 1, DAP kinase 1, D Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of DAP Kinase 1 (DAPK1) Antibody (C-term) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The DAPK1 polyclonal antibody is used in Western blot to detect DAPK1 in mouse liver tissue lysate.