DAP3 Antibody

• Catalog Number: orb1247986
• Category: Antibodies
• Description: DAP3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: NPSLLERHCAYL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1ug/ml. Primary incubated 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DAP3
• UniProt ID: P51398
• NCBI: NP_387506.1, NP_001186780.1, NP_001186779.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DAP3, death associated protein 3, DAP-3, DKFZp686G
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1ug/ml. Primary incubated 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247986 (0.3 ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.

orb1247986 (1 ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR.

orb1247986 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247986 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247986 staining (0.3 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.

orb1247986 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).