DAGLA Antibody

• Catalog Number: orb1246561
• Category: Antibodies
• Description: DAGLA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PAKQDELVISAR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration: 0.3-1ug/ml. Some minor background is detected and is blocked by the immunizing peptide. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:Paraffin embedded Human Breast, Pancreas and Spleen. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DAGLA
• UniProt ID: Q9Y4D2
• NCBI: NP_006124.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DAGLA, diacylglycerol lipase, alpha, C11orf11, DAG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration: 0.3-1ug/ml. Some minor background is detected and is blocked by the immunizing peptide. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:Paraffin embedded Human Breast, Pancreas and Spleen. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246561 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246561 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.