DAD1 Antibody

• Catalog Number: orb1239920
• Category: Antibodies
• Description: DAD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.The immunogen is located within the last 50 amino acids of DAD1.
• Concentration: 1 mg/mL
• Dilution range: DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DAD1
• UniProt ID: P61803
• NCBI: AAH09798
• Storage: DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DAD1 Antibody: OST2, Dolichyl-diphosphooligosaccha
• Note: For research use only
• Application notes: DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 µg/mL.

Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 µg/mL.