TPD52L2 Antibody

SKU: orb1247626

Description

TPD52L2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. See also our D52 and D53 products.Immunohistochemistry:Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75µg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SGDKPLSDPAP
• Target: TPD52L2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TPD52L2, D54, tumor protein D52-like 2 , DKFZp686A1765, hD54, HCCR-binding protein 2

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247626 (0.1 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247626 (3.75 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TPD52L2

UniProt

O43399

RefSeq (Protein):NP_955395.1, NP_955392.1, NP_955391.1, NP_003279.2, NP_955393.1, NP_955394.1