DAO Antibody

SKU: orb345020

Description

D-Amino Acid Oxidase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  D-AMINO ACID OXIDASE antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of D-Amino Acid Oxidase [Pig Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: D-Amino Acid Oxidase [Pig Kidney]
• Target: DAO
• Purity: Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney]. Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO

Western Blot of Sheep anti-D-Amino Acid Oxidase Antibody. Lane 1: D-Amino Acid Oxidase. Lane 2: None. Load: 50 ng per lane. Primary antibody: D-Amino Acid Oxidase primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 39 kDa, 39 kDa for D-Amino Acid Oxidase. Other band(s): None.

Quick Database Links

Gene Symbol

DAO

UniProt

P00371

RefSeq (Protein):NP_999231.1