Cytokeratin 7 Rabbit mAb [KD Validated] Antibody

SKU: orb1951645

Description

K7 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Cytokeratin 7
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK-7; CK7; cytokeratin 7; Cytokeratin-7; K2C7; K7; keratin 7; Keratin-7; KRT7; Sarcolectin; SCL;

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using Cytokeratin 7 Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cytokeratin 7 Antibody.

Western blot analysis of Cytokeratin 7 expression in T47 D cell lysate.

Quick Database Links

UniProt

P08729