KRT7 Antibody (Biotin)

SKU: orb750864

Description

Mouse monoclonal antibody against KRT7 conjugated to Biotin

Research Area

Cell Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix, or breast.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: OV-TL12/30
• Immunogen: OTN 11, ovarian carcinoma cell line
• Target: KRT7
• Source/Expression System: Bioreactor
• Molecular Weight: 55kDa
• Purification: Protein G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CK-7 antibody, anti-K2C7 antibody, anti-Keratin 55K Type II Cytoskeletal antibody, anti-Keratin Simple Epithelial Type 1 K7 antibody, anti-Keratin Type II Cytoskeletal 7 antibody, anti-Krt2-7 antibody, anti-KRT7 antibody, anti-Sarcolectin antibody, anti-SCL antibody, anti-Type II Mesothelial Keratin K7 antibody, anti-Type-II Keratin Kb7 antibody

Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of methanol-fixed HeLa cells. KRT7 Mouse Monoclonal Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

SDS-PAGE Analysis Purified Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

KRT7

UniProt

P08729

NCBI Gene ID

3855