Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

• Catalog Number: orb750864
• Category: Antibodies
• Description: Mouse monoclonal antibody against KRT7 conjugated to Biotin
• Target: KRT7
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein G
• Immunogen: OTN 11, ovarian carcinoma cell line
• UniProt ID: P08729
• MW: 55kDa
• Tested applications: FC, IF, IHC, WB
• Dilution range: Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Western Blot (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix, or breast.
• Clone Number: OV-TL12/30
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CK-7 antibody, anti-K2C7 antibody, anti-Kerat
• Research Area: Cell Biology, Stem Cell & Developmental Biology
• Note: For research use only
• Entrez: 3855
• Expiration Date: 12 months from date of receipt.

Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (SPM270) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of methanol-fixed HeLa cells. KRT7 Mouse Monoclonal Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

SDS-PAGE Analysis Purified Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30). Confirmation of Integrity and Purity of Antibody.