KRT7 Antibody

SKU: orb1252502

Description

KRT7 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: OV-TL12/30
• Immunogen: OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.
• Target: KRT7
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL

Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (OV-TL12/30).

Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (OV-TL12/30).

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone OV-TL12/30); Red = isotype control, Blue= recombinant Cytokeratin 7 antibody.

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone OV-TL12/30).

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.

Quick Database Links

Gene Symbol

KRT7

UniProt

P08729