Cytokeratin 5/6, CK5/6 Antibody

SKU: orb1294308

Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:300
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Target: Cytokeratin 5/6, CK5/6
• Molecular Weight: 43-55 kDa
• Purity: Affinity column purified

Storage & Handling

• Storage: Store at 4°C for short-term storage. Long-time storage is recommended at -20°C
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Concentration: 1.74 mg/ml
• Disclaimer: For research use only

All lanes: Anti-Cytokeratin 5/6 antibody - at 1/2000 dilution, Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294308 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294308 anti-Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294308 anti-Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 5/6 with orb1294308 at 1/100.

Quick Database Links

Gene Symbol

Cytokeratin 5/6, CK5/6