Cytokeratin 20 Antibody / CK20

• Catalog Number: orb1151515
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Cytokeratin 20
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 196-323 was used as the immunogen for the recombinant Keratin 20 antibody.
• UniProt ID: P35900
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Application notes: Optimal dilution of the recombinant Keratin 20 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: KRT20/4879R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only

IHC staining of FFPE human colon tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. Inset: PBS instead of primary Ab to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Keratin 20 antibody (clone KRT20/4879R) as confirmation of integrity and purity.