Cytokeratin 19 antibody

SKU: orb2276557

Description

Cytokeratin 19 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A strong immunostaining is seen in all epithelial cells. Tonsil: A moderate to strong immunostaining occurs in the basal cell layer of the squamous epithelium of the tonsil surface. Negative Control: Colon: All non-epithelial cells are CK19 negative. Tonsil: All non-basal squamous epithelial cells and all non-epithelial cells are CK19 negative. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

  Read more...

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, lambda + IgG1, kappa
• Clone No.: MSVA-119M
• Immunogen: Recombinant human KRT19 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

k19; k1cs; Keratin 19 Keratin Type i 40kD; krt19

Quick Database Links

UniProt

P08727