Cytokeratin 18 Antibody

SKU: orb2638774

Description

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the Cytokeratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KRT18/835
• Immunogen: Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone KRT18/835).

IHC: Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Cytokeratin 18 antibody (KRT18/835).

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 18 antibody (clone KRT18/835).

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 antibody (clone KRT18/835).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 18 antibody (KRT18/835).

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (green, clone KRT18/835) and Reddot nuclear stain (red).

Western blot testing of HCT116 cell lysate with Cytokerain 18 antibody (clone KRT18/835). Expected molecular weight: 46-50 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Cytokeratin 18 antibody (clone KRT18/835). These results demonstrate the foremost specificity of the KRT18/835 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone KRT18/835) as confirmation of integrity and purity.

Quick Database Links

UniProt

P05783