Keratin 18 Antibody

SKU: orb2638765

Description

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the Keratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KRT18/1190
• Immunogen: Recombinant human KRT18 protein was used as the immunogen for the Keratin 18 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human HeLa cells with Keratin 18 antibody (clone KRT18/1190, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Keratin 18 antibody (clone KRT18/1190).

IHC: Formalin-fixed, paraffin-embedded rat colon stained with Keratin 18 antibody (clone KRT18/1190).

Western blot testing of human 1) stomach and 2) intestine lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.

Western blot testing of human HCT116 cell lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.

Western blot testing of human A549 and HEP-2 cell lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.

Quick Database Links

UniProt

P05783