Cytokeratin 18 Antibody

• Catalog Number: orb749636
• Category: Antibodies
• Description: This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A cytoskeletal preparation from A431 cells was used as the immunogen for the Cytokeratin 18 antibody.
• UniProt ID: P05783
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Cytokeratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: DE-K18
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This Cytokeratin 18 antibody is available for research use only.
• Note: For research use only

Flow cytometry testing of MeOH fixed human HeLa cells with Cytokeratin 18 antibody (clone DE-K18); Red = isotype control, Blue = Cytokeratin 18 antibody.

IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (clone DE-K18).

Immunofluorescent staining of permeabilized human HeLa cells with Cytokeratin 18 antibody (green, clone DE-K18) and Reddot nuclear stain (red).

Western blot testing of MCF7 cell lysate with Cytokerain 18 antibody (clone DE-K18). Expected molecular weight: 46-50 kDa.

Western blot testing of HCT116 cell lysate with Cytokerain 18 antibody (clone DE-K18). Expected molecular weight: 46-50 kDa.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 18 antibody (clone DE-K18) as confirmation of integrity and purity.