Cytokeratin 18 Antibody

SKU: orb385859

Description

Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the Cytokeratin 18 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CTKN18-1
• Immunogen: Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 18 antibody (clone CTKN18-1, green) and Reddot nuclear stain (red).

IHC testing of FFPE human lung carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.

IHC testing of FFPE human thyroid carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.

IHC testing of FFPE human colon carcinoma and Cytokeratin 18 antibody (clone CTKN18-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 min, followed by cooling at RT for 20 min, prior to staining.

Western blot testing of HCT-116 cell lysate with Cytokeratin 18 antibody (clone CTKN18-1). Predicted molecular weight ~48 kDa.

Quick Database Links

UniProt

P05783