Cytokeratin 18 Antibody Cocktail

SKU: orb639475

Description

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Flow Cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Cytokeratin 18 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: Clone Cocktail
• Immunogen: Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody cocktail.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 18 antibody.

IHC: Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Cytokeratin 18 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 antibody.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody.

IHC: Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 18 antibody.

Western blot testing of human HCT116 cells with Cytokeratin 18 antibody cocktail. Predicted molecular weight ~45 kDa.

Flow cytometry staining of PFA-fixed human HeLa cells with Cytokeratin 18 antibody cocktail; Red = isotype control, Blue = Cytokeratin 18 antibody.

Quick Database Links

UniProt

P05783