Cytokeratin 17 Antibody

SKU: orb385874

Description

Cytokeratin 17 is a type I keratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. [Wiki]

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
• Reactivity: Human
• Application Notes:
  Titering of the Cytokeratin 17 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: CTKN17-1
• Immunogen: Recombinant full-length human KRT17 protein was used as the immunogen for the Cytokeratin 17 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE cervical carcinoma and Cytokeratin 17 antibody (clone CTKN17-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE bladder carcinoma and Cytokeratin 17 antibody (clone CTKN17-1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Quick Database Links

UniProt

Q04695