Cytokeratin 7/17 Antibody

SKU: orb43713

Description

Mouse Monoclonal to Cytokeratin 7/17.

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Reactivity: Bovine, Human, Porcine
• Application Notes:
  Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: C-46
• Immunogen: Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
• Target: Cytokeratin 7/17
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7

Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7+17 (C-46).

Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).

Western blotting analysis of human cytokeratin 7+17 using mouse monoclonal antibody C-46 on lysates of HeLa cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 7+17 monoclonal antibody C-46 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.

Quick Database Links

Gene Symbol

Cytokeratin 7/17

NCBI Gene ID

35