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Item 1 of 3
Anti-Cytokeratin 7/17 Purified
Catalog Number: orb43713
| Catalog Number | orb43713 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Cytokeratin 7/17. |
| Clonality | Monoclonal |
| Clone Number | C-46 |
| Tested applications | ICC, IHC-P, IP, WB |
| Reactivity | Bovine, Human, Porcine |
| Isotype | Mouse IgG1 |
| Immunogen | Cytoskeleton preparation of HeLa human cervix carcinoma cell line. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | Cytokeratin 7/17 |
| RRID | AB_10996820 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-Cytokeratin 7+17 antibody Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7+17 (C-46).
Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).
Western blotting analysis of human cytokeratin 7+17 using mouse monoclonal antibody C-46 on lysates of HeLa cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 7+17 monoclonal antibody C-46 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.
