Cytokeratin 15 Mouse Monoclonal Antibody

SKU: orb1602368

Description

Cytokeratin 15 Mouse Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, Kappa
• Clone No.: 2H8
• Immunogen: KLH conjugated synthetic peptide derived from human Cytokeratin 15
• Target: KRT15
• Molecular Weight: 49 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid in PBS containing 50% Glycerol, 0.5% rAlbumin and 0.02% Proclin300.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

K1C15_HUMAN; Cytokeratin-15; CK 15; CK-15; CK15; K15; K1CO; Keratin 15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; Keratin15; KRT 15; KRTB; KRTL15; keratin-15, basic; Type I cytoskeletal 15.

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (orb1602368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (orb1602368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (orb1602368) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

KRT15

UniProt

P19012