KRT14 Antibody (Biotin)

SKU: orb751277

Description

Mouse monoclonal antibody against KRT14 conjugated to Biotin

Research Area

Cell Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution Range: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: HeLa, A549, or A431 cells. Skin or squamous cell carcinoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone No.: LL002
• Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
• Target: KRT14
• Source/Expression System: Bioreactor
• Molecular Weight: ~50kDa
• Purification: Protein G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CK-14 antibody, anti-Dowling Meara antibody, anti-ebs3 antibody, anti-ebs4 antibody, anti-Epidermolysis Bullosa Simplex antibody, anti-Keratin Type I Cytoskeletal 14 antibody, anti-Koebner antibody, anti-NFJ antibody

Quick Database Links

Gene Symbol

KRT14

UniProt

P02533

NCBI Gene ID

3861