KRT14 Antibody

SKU: orb1252735

Description

KRT14 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 1-2 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone No.: LL002
• Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.
• Target: KRT14
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14

IHC testing of human prostate (10X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of human prostate (20X) stained with Cytokeratin 14 antibody (LL002).

IHC testing of FFPE human prostate with Cytokeratin 14 antibody.

IHC testing of FFPE human prostate with Cytokeratin 14 antibody.

Western blot testing of A431 cell lysate (epidermoid carcinoma) and Cytokeratin 14 antibody (clone LL002) at 1 ug/ml. Predicted/observed molecular weight: ~53kDa.

Quick Database Links

Gene Symbol

KRT14

UniProt

P02533